Research Interest

• Amyloids, Alzheimer's disease, Neurodegeneration
• Protein misfolding, aggregation and proteostasis
• Mass spectrometry and proteomics
• Autophagy and UPS
• Ubiquitination
• Cancer
• Aging

  Academic Background

• Ph.D.: Indian Institute of Technology Jodhpur- Department of Bioscience and Bioengineering
• M.Sc.: University of Lucknow- Department of Biochemistry
• B.Sc. Honors: University of Delhi- Hans Raj College

  Professional Experience

• Adjunct Faculty, Northwestern University Feinberg School of Medicine Chicago (2024- Till now)
• Postdoctoral Fellow, Northwestern University Feinberg School of Medicine Chicago (2019- 2024)
• Assistant Professor, Central University of Rajasthan, Depertment of Biochemistry (2018- 2019)

  Webpage

• Personal Website

• Faculty Profile

  Scientific Contributions and Outreach

• Associate Editor of BMC Molecular and Cell Biology
• Associate Editor of BMC Neuroscience
• Associate Editor of BMC Biotechnology
• Associate Editor Frontiers in Genetics
• Academic Editor Plos One

  Memberships

• The Alzheimer's Association-International Society to Advance Alzheimer's Research and Treatment (ISTAART)
• International Society for Molecular Neurodegeneration (ISMND)
• International Parkinson and Movement Disorder Society
• American Neurological Association (ANA)
• Biochemical Society UK
• Genetics Society of America (GSA- Presidential membership award)
• American Association for Cancer Research (AACR)
• Society for Neuroscience (SfN)
• American Society of Mass Spectrometry (ASMS)
• American Society for Microbiology (ASM)
• European Society for Medical Oncology (ESMO)
• Global Association for the Study of Neurodegenerative Diseases, INC. (GASND)
• Indian Society for Chemists and Biologists
• Indian Academy of Neurosciences (IAN)
• Indian Society for Cell Biology

  PUBLICATIONS

• Notable Research Publications
• Rao NR*, Upadhyay A*, Savas JN. Derailed protein turnover in the aging mammalian brain. Molecular Systems Biology. 2024;20(2):120-39. *equal contribution
• Upadhyay A, Chhangani D, Nalini R. Rao, Julia Kofler, Robert Vassar, Diego E. Rincon-Limas, Jeffrey N. Savas. Amyloid fibril proteomics of AD brains reveals modifiers of aggregation and toxicity. Molecular Neurodegeneration, 2023;18(61).
• Rao NR, Upadhyay A, Savas JN, et al. Levetiracetam prevents Aβ42 production through SV2a-dependent modulation of App processing in Alzheimer's disease models, bioArxiv 2024.
• Upadhyay A, Vassar RJ, Savas JN. Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain. JoVE (Journal of Visualized Experiments). 2022(182):e63816.
• Upadhyay A, Amanullah A, Mishra R, Kumar A, Mishra A. Lanosterol Suppresses the Aggregation and Cytotoxicity of Misfolded Proteins Linked with Neurodegenerative Diseases. Mol. Neurobiology. 2017:1-14.
• Upadhyay A, Amanullah A, Chhangani D, Joshi V, Mishra R, Mishra A. Ibuprofen induces mitochondrial-mediated apoptosis through proteasomal dysfunction. Molecular neurobiology. 2016;53:6968-81.
• Highly-cited Reviews
• Singh S, Joshi V, Upadhyay A*. Amyloids and brain cancer: molecular linkages and crossovers. Bioscience Reports. 2023;43(10):BSR20230489. *corresponding author
• Upadhyay A*, Sundaria N*, Dhiman R, Prajapati VK, Prasad A, Mishra A. Complex inclusion bodies and defective proteome hubs in neurodegenerative disease: new clues, new challenges. The Neuroscientist. 2022;28(3):271-82. *equal contribution
• Anand U, Dey A, Chandel AKS, Sanyal R, Mishra A, Pandey DK, Upadhyay A, et al. Cancer chemotherapy and beyond: Current status, drug candidates, associated risks and progress in targeted therapeutics. Genes & Diseases. 2022.
• Upadhyay A, Amanullah A, Joshi V, Dhiman R, Prajapati VK, Poluri KM, et al. Ibuprofen-based advanced therapeutics: breaking the inflammatory link in cancer, neurodegeneration, and diseases. Drug Metabolism Reviews. 2021;53(1):100-21
• Upadhyay A. Natural compounds in the regulation of proteostatic pathways: An invincible artillery against stress, ageing, and diseases. Acta Pharmaceutica Sinica B. 2021;11(10):2995-3014. *corresponding author
• Upadhyay A. Cancer: An unknown territory; rethinking before going ahead. Genes & Diseases. 2021;8(5):655-61. *corresponding author
• Sundaria N*, Upadhyay A*, Prasad A, Prajapati VK, Poluri KM, Mishra A. Neurodegeneration & imperfect ageing: Technological limitations and challenges? Mechanisms of Ageing and Development. 2021; 200:111574. *equal contribution
• Prasanna P, Upadhyay A. Flavonoid-Based Nanomedicines in Alzheimer’s Disease Therapeutics: Promises Made, a Long Way To Go. ACS Pharmacology & Translational Science. 2021;4(1):74-95. *corresponding author
• Prasanna P, Upadhyay A. Heat shock proteins as the druggable targets in leishmaniasis: promises and perils. Infection and immunity. 2021;89(2):10.1128/iai. 00559-20. *corresponding author
• Joshi V, Upadhyay A, Prajapati VK, Mishra A. How autophagy can restore proteostasis defects in multiple diseases? Medicinal research reviews. 2020;40(4):1385-439.
• Graykowski DR, Wang Y-Z, Upadhyay A, Savas JN. The dichotomous role of extracellular vesicles in the central nervous system. Iscience. 2020;23(9).
• Upadhyay A. Structure of proteins: Evolution with unsolved mysteries. Progress in Biophysics and Molecular Biology. 2019. *corresponding author
• Mishra R, Upadhyay A, Prajapati VK, Dhiman R, Poluri KM, Jana NR, et al. LRSAM1 E3 ubiquitin ligase: molecular neurobiological perspectives linked with brain diseases. Cellular and Molecular Life Sciences. 2019;76:2093-110.
• Upadhyay A, Mishra A. Amyloids of multiple species: are they helpful in survival? Biological Reviews. 2018;93(3):1363-86.
• Upadhyay A, Joshi V, Amanullah A, Mishra R, Arora N, Prasad A, et al. E3 Ubiquitin Ligases Neurobiological Mechanisms: Development to Degeneration. Frontiers in Molecular Neuroscience. 2017;10:151.
• Amanullah A, Upadhyay A, Joshi V, Mishra R, Jana NR, Mishra A. Progressing neurobiological strategies against proteostasis failure: Challenges in neurodegeneration. Progress in neurobiology. 2017;159:1-38.
• Upadhyay A, Amanullah A, Chhangani D, Mishra R, Prasad A, Mishra A. Mahogunin ring finger-1 (MGRN1), a multifaceted ubiquitin ligase: recent unraveling of neurobiological mechanisms. Molecular neurobiology. 2016;53(7):4484-96
• Upadhyay A, Amanullah A, Chhangani D, Mishra R, Mishra A. Selective multifaceted E3 ubiquitin ligases barricade extreme defense: Potential therapeutic targets for neurodegeneration and ageing. Ageing Research Reviews. 2015;24:138-59.
• Other notable contributions
• Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, et al. Pulse-chase proteomics of the App knockin mouse models of Alzheimer’s disease reveals that synaptic dysfunction originates in presynaptic terminals. Cell systems. 2021;12(2):141-58. e9.
• Mishra R, Joshi V, Upadhyay A, Amanullah A, Dubey AR, Singh S, et al. LRSAM1 E3 ubiquitin ligase promotes proteasomal clearance of E6-AP protein. Cellular Signalling. 2021;77:109836.
• Prasanna P, Kumar R, Singh VK, Upadhyay A. Cloning, purification, and homology modeling of Histone deacetylase in Leishmania donovani. Infection, Genetics and Evolution. 2021;89:104738.
• Mishra R, Amanullah A, Upadhyay A, Dhiman R, Dubey AR, Singh S, et al. Ubiquitin ligase LRSAM1 suppresses neurodegenerative diseases linked aberrant proteins induced cell death. The International Journal of Biochemistry & Cell Biology. 2020;120:105697.>
• Sherine J, Upadhyay A, Mishra A, Kumar D, Pal S, Harinipriya S. Ag (I) and Au (III) Mercaptobenzothiazole complexes induced apoptotic cell death. Scientific Reports. 2019;9(1):621.
• Joshi V, Mishra R, Upadhyay A, Amanullah A, Poluri KM, Singh S, et al. Polyphenolic flavonoid (Myricetin) upregulated proteasomal degradation mechanisms: Eliminates neurodegenerative proteins aggregation. Journal of cellular physiology. 2019;234(11):20900-14.
• Joshi V, Upadhyay A, Chhangani D, Amanullah A, Sharan RN, Mishra A. Gp78 involvement in cellular proliferation: Can act as a promising modulator for cell cycle regulatory proteins? Journal of cellular physiology. 2018;233(10):6352-68.
• Khan E, Tawani A, Mishra SK, Verma AK, Upadhyay A, Kumar M, et al. Myricetin reduces toxic level of CAG repeats RNA in Huntington’s disease (HD) and Spino cerebellar Ataxia (SCAs). ACS chemical biology. 2018;13(1):180-8.
• Awasthi M, Upadhyay A, Singh S, Pandey VP, Dwivedi UN. Terpenoids as promising therapeutic molecules against Alzheimer’s disease: amyloid beta-and acetylcholinesterase-directed pharmacokinetic and molecular docking analyses. Molecular Simulation. 2018;44(1):1-11.
• Amanullah A, Mishra R, Upadhyay A, Reddy PP, Das R, Mishra A. Indomethacin elicits proteasomal dysfunctions develops apoptosis through mitochondrial abnormalities. Journal of cellular physiology. 2018;233(2):1685-99.
• Amanullah A, Upadhyay A, Chhangani D, Joshi V, Mishra R, Yamanaka K, et al. Proteasomal Dysfunction Induced By Diclofenac Engenders Apoptosis Through Mitochondrial Pathway. Journal of cellular biochemistry. 2017;118(5):1014-27.
• Chhangani D, Endo F, Amanullah A, Upadhyay A, Watanabe S, Mishra R, et al. Mahogunin ring finger 1 confers cytoprotection against mutant SOD1 aggresomes and is defective in an ALS mouse model. Neurobiology of disease. 2016;86:16-28.
• Chhangani D, Upadhyay A, Amanullah A, Joshi V, Mishra A. Ubiquitin ligase ITCH recruitment suppresses the aggregation and cellular toxicity of cytoplasmic misfolded proteins. Scientific reports. 2014;4(1):5077.
• Chhangani D, Nukina N, Kurosawa M, Amanullah A, Joshi V, Upadhyay A, et al. Mahogunin ring finger 1 suppresses misfolded polyglutamine aggregation and cytotoxicity. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2014;1842(9):1472-84.
• Book Chapters
• Upadhyay A., Vibhuti Joshi. Springer Nature; 2024. Proteasome activators and aging: restoring proteostasis using small molecules, in Book: Biochemistry and Cell Biology of Ageing: Part V, Anti-Ageing Interventions, 2024, Springer Nature, *corresponding author
• Aggarwal V., Rathod S., Vashishtha K., Upadhyay A. Immune Cell Metabolites as Fuel for Cancer Cells, in Book: Immuno-Oncology Crosstalk and Metabolism, 153-186, 2022. Springer Nature.
• Rathod S., Aggarwal V., Upadhyay A., Choudhari R. Timing of the Major Metabolic Switches in Immune Cell Activation and Differentiation During Cancer Development Book: Immuno-Oncology Crosstalk and Metabolism, 187-218, 2022. Springer Nature.
• Badhwar R, Upadhyay A. Modulation of Cellular Protein Quality Control Pathways Using Small Natural Molecules. Bioactive Natural products in Drug Discovery. 609-41, 2020, Springer Nature. *corresponding author
• Joshi V, Upadhyay A, Amanullah A, Mishra R, Mishra A. Predicting E3 Ubiquitin Ligases as Possible Promising Biomarkers for Brain Tumors. In Book: Molecular Diagnostics in Cancer Patients, 43-72, 2019, Springer.
• Upadhyay A, Amanullah A, Joshi V, Mishra R, Mishra A. Molecular and Cellular Insights: Neuroinflammation and Amyotrophic Lateral Sclerosis. In Book: Inflammation: The Common Link in Brain Pathologies: 209-30, 2016, Springer.
• Dwivedi U., Awasthi M., Upadhyay A., Singh S., Pandey VP. An overview of progression and treatment of Amyloid Beta dependent Alzheimer’s Diseases Book: Public Health and Development in India. 2016 Northern Book Centre, New Delhi

  Scholary Profiles

• Google Scholar Profile
• ORCID Profile
• VIDWAN ID